Identification and Quantitation of Cashmere (Pashmina) Fiber and Wool Using Novel Microchip Based Real-Time PCR Technology
نویسندگان
چکیده
منابع مشابه
Quantitation of HCV RNA using real-time PCR and fluorimetry.
Real-time PCR technology may provide an accurate and sensitive method to quantify hepatitis C virus (HCV) RNA. So far, studies have been carried out using the Taqman technology with the ABI Prism 7700 sequence detector. An alternative and simple real-time PCR assay is described with no probe requirement, based on the SYBR Green I dye and LightCycler fluorimeter. Amplicon synthesis was monitored...
متن کاملReal-Time Building Information Modeling (BIM) Synchronization Using Radio Frequency Identification Technology and Cloud Computing System
The online observation of a construction site and processes bears significant advantage to all business sector. BIM is the combination of a 3D model of the project and a project-planning program which improves the project planning model by up to 6D (Adding Time, Cost and Material Information dimensions to the model). RFID technology is an appropriate information synchronization tool between the...
متن کاملReal-time PCR for mRNA quantitation.
Real-time PCR has become one of the most widely used methods of gene quantitation because it has a large dynamic range, boasts tremendous sensitivity, can be highly sequence-specific, has little to no post-amplification processing, and is amenable to increasing sample throughput. However, optimal benefit from these advantages requires a clear understanding of the many options available for runn...
متن کاملDetection of Fuel Adulteration in Real Time Using Optical Fiber Sensor and Peripheral Interface Controller
Detection of fuel adulteration in real time is the most important need of the world as it generates the hazardous effects on the environment, living and nonliving things. Various automotive users and fuel providers may mix kerosene in petrol and diesel. Adulterated fuel increases air pollution, as well as reduces the performance of vehicle engines. Such a malpractice in fuel is prevailing becau...
متن کاملMicrochip-based one step DNA extraction and real-time PCR in one chamber for rapid pathogen identification.
Optimal detection of a pathogen present in biological samples depends on the ability to extract DNA molecules rapidly and efficiently. In this paper, we report a novel method for efficient DNA extraction and subsequent real-time detection in a single microchip by combining laser irradiation and magnetic beads. By using a 808 nm laser and carboxyl-terminated magnetic beads, we demonstrate that a...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Textile Science and Technology
سال: 2018
ISSN: 2379-1543,2379-1551
DOI: 10.4236/jtst.2018.44010